Composite

Part:BBa_K4335020:Design

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)


HSP70A Promoter+Flag+NLS+SpCas9+NLS+Rbcs2 Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 466
    Illegal PstI site found at 1012
    Illegal PstI site found at 2434
    Illegal PstI site found at 2638
    Illegal PstI site found at 2668
    Illegal PstI site found at 3880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
    Illegal PstI site found at 466
    Illegal PstI site found at 1012
    Illegal PstI site found at 2434
    Illegal PstI site found at 2638
    Illegal PstI site found at 2668
    Illegal PstI site found at 3880
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 473
    Illegal BglII site found at 1268
    Illegal BglII site found at 1958
    Illegal BglII site found at 4037
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 466
    Illegal PstI site found at 1012
    Illegal PstI site found at 2434
    Illegal PstI site found at 2638
    Illegal PstI site found at 2668
    Illegal PstI site found at 3880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 466
    Illegal PstI site found at 1012
    Illegal PstI site found at 2434
    Illegal PstI site found at 2638
    Illegal PstI site found at 2668
    Illegal PstI site found at 3880
    Illegal NgoMIV site found at 1300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3693


Design Notes

Source

References